Menu 
  • Home
  • Research on Station
        • Benefits of Research on the ISS
        • Industrial R&D
        • Current Project Pipeline
        • Researcher Interviews
      • Current RFI

        hardware

        RFI-Organs-On-Chips Research

      • Researcher Interviews

        No items found
  • Getting to Space
        • Getting to Space
        • Implementation Partners
        • ISS Hardware
        • Proposal Submission Process
        • Launch Vehicles
        • Support Services
      • Recent Posts

        No items found
      • Projects in Flight

        • Windows On Earth
        • Street View Imagery Collect on ISS
        • Cyclone Intensity Measurements from the International Space Station (CIMISS)
        • Materials Testing – Earth Abundant Textured Thin Film Photovoltaics
        • GLASS AIS TransponderGlobal AIS on Space Station (GLASS)
        View Current ISS Project Pipeline »
  • Research Library
        • ISS National Lab Research Database
        • ISS National Lab Reports
        • Web Resources
        • Research Apps
      • Recently Added Research

        • Genotype, B-vitamin status, and androgens affect spaceflight-induced ophthalmic changes
        • SUBSONIC MOTION OF A PROJECTILE IN A FLUID COMPLEX PLASMA UNDER MICROGRAVITY CONDITIONS
        • Coactivator-Dependent Oscillation of Chromatin Accessibility Dictates Circadian Gene Amplitude via REV-ERB Loading
      • Popular Tags

        • Cell Differentiation
        • Earth Observation
        • Fluid physics
        • Gene Expression
        • Human Research
        • Material science
        • Mice
        • Microbiology
        • Simulated microgravity
        • Technology demonstration
  • Make Contact
  • Home
  • Research on Station
    • Benefits of Microgravity
    • Industrial R&D
    • Current Project Pipeline
    • Research Opportunities
    • Researcher Interviews
  • Facilities & Hardware
    • ISS Hardware
    • Implementation Partners
  • Getting to Space
    • Getting to Space
    • Proposal Submission Process
    • Launch Vehicles
  • Research Library
    • Research Apps
    • Researcher Guides
    • Resources
    • Publication Database

« Go Back

Simulated Microgravity Maintains the Undifferentiated State and Enhances the Neural Repair Potential of Bone Marrow Stromal Cells

Yuge, L., et al. (2011). "Simulated Microgravity Maintains the Undifferentiated State and Enhances the Neural Repair Potential of Bone Marrow Stromal Cells." Stem Cells and Development 20 5: 893-900

Recently, regenerative medicine with bone marrow stromal cells (BMSCs) has gained significant attention for the treatment of central nervous system diseases. Here, we investigated the activity of BMSCs under simulated microgravity conditions. Mouse BMSCs (mBMSCs) were isolated from C57BL/6 mice and harvested in 1G condition. Subjects were divided into 4 groups: cultured under simulated microgravity and 1G condition in growth medium and neural differentiation medium. After 7 days of culture, the mBMSCs were used for morphological analysis, reverse transcription (RT)-polymerase chain reaction, immunostaining analysis, and grafting. Neural-induced mBMSCs cultured under 1G conditions exhibited neural differentiation, whereas those cultured under simulated microgravity did not. Moreover, under simulated microgravity conditions, mBMSCs could be cultured in an undifferentiated state. Next, we intravenously injected cells into a mouse model of cerebral contusion. Graft mBMSCs cultured under simulated microgravity exhibited greater survival in the damaged region, and the motor function of the grafted mice improved significantly. mBMSCs cultured under simulated microgravity expressed CXCR4 on their cell membrane. Our study indicates that culturing cells under simulated microgravity enhances their survival rate by maintaining an undifferentiated state of cells, making this a potentially attractive method for culturing donor cells to be used in grafting.

Related URLs:
<Go to ISI>://WOS:000290255300013

ISSN: 1547-3287

DOI: 10.1089/scd.2010.0294

Accession Number: WOS:000290255300013

Share this
0
0
0
Tags: Animal, Animals, Bone Marrow Cells/*cytology/metabolism, Bone Marrow Transplantation, Cell Culture Techniques/*methods, Cell Differentiation, Cell Survival, Cells, Cultured, Inbred C57BL, Mice, Models, Receptors, Regenerative medicine, Stromal Cells/*cytology/metabolism, Weightlessness