Menu 
  • Home
  • Research on Station
        • Benefits of Research on the ISS
        • Industrial R&D
        • Current Project Pipeline
        • Researcher Interviews
      • Current RFI

        hardware

        RFI-Organs-On-Chips Research

      • Researcher Interviews

        No items found
  • Getting to Space
        • Getting to Space
        • Implementation Partners
        • ISS Hardware
        • Proposal Submission Process
        • Launch Vehicles
        • Support Services
      • Recent Posts

        No items found
      • Projects in Flight

        • Street View Imagery Collect on ISS
        • Cyclone Intensity Measurements from the International Space Station (CIMISS)
        • Materials Testing – Earth Abundant Textured Thin Film Photovoltaics
        • GLASS AIS TransponderGlobal AIS on Space Station (GLASS)
        • MultiLab: Research Server for the ISS
        View Current ISS Project Pipeline ยป
  • Research Library
        • ISS National Lab Research Database
        • ISS National Lab Reports
        • Web Resources
        • Research Apps
      • Recently Added Research

        • Genotype, B-vitamin status, and androgens affect spaceflight-induced ophthalmic changes
        • SUBSONIC MOTION OF A PROJECTILE IN A FLUID COMPLEX PLASMA UNDER MICROGRAVITY CONDITIONS
        • Coactivator-Dependent Oscillation of Chromatin Accessibility Dictates Circadian Gene Amplitude via REV-ERB Loading
      • Popular Tags

        • Cell Differentiation
        • Earth Observation
        • Fluid physics
        • Gene Expression
        • Human Research
        • Material science
        • Mice
        • Microbiology
        • Simulated microgravity
        • Technology demonstration
  • Make Contact
  • Home
  • Research on Station
    • Benefits of Microgravity
    • Industrial R&D
    • Current Project Pipeline
    • Research Opportunities
    • Researcher Interviews
  • Facilities & Hardware
    • ISS Hardware
    • Implementation Partners
  • Getting to Space
    • Getting to Space
    • Proposal Submission Process
    • Launch Vehicles
  • Research Library
    • Research Apps
    • Researcher Guides
    • Resources
    • Publication Database

To investigate the effects of microgravity on murine skeletal muscle fiber size, muscle contractile protein, and enzymatic activity, female C57BL/6J mice, aged 64 days, were divided into animal enclosure module (AEM) ground control and spaceflight (SF) treatment groups. SF animals were flown on the space shuttle Endeavour (STS-108/UF-1) and subjected to approximately 11 days and 19 h of microgravity. Immunohistochemical analysis of muscle fiber cross-sectional area revealed that, in each of the muscles analyzed, mean muscle fiber cross-sectional area was significantly reduced (P < 0.0001) for all fiber types for SF vs. AEM control. In the soleus, immunohistochemical analysis of myosin heavy chain (MHC) isoform expression revealed a significant increase in the percentage of muscle fibers expressing MHC IIx and MHC IIb (P < 0.05). For the gastrocnemius and plantaris, no significant changes in MHC isoform expression were observed. For the muscles analyzed, no alterations in MHC I or MHC IIa protein expression were observed. Enzymatic analysis of the gastrocnemius revealed a significant decrease in citrate synthase activity in SF vs. AEM control.

Related URLs:
http://www.ncbi.nlm.nih.gov/pubmed/12882990

Share this
0
0
0
Tags: Adaptation, Animals, Body Weight/physiology, Citrate (si)-Synthase/metabolism, cytology, Female, Heart/anatomy & histology, Immunohistochemistry, Inbred C57BL, International Space Station, Isomerism, Mice, Muscle, Muscle Fibers, Myosin Heavy Chains/biosynthesis/metabolism, Organ Size/physiology, Oxidation-Reduction, Physiological/*physiology, Skeletal/cytology/metabolism/*physiology, Skeletal/ultrastructure, Space Flight, Weightlessness/*adverse effects