Spaceflight and simulated microgravity cause a significant reduction of key gene expression in early T-cell activation
Martinez, E. M., et al. (2015). "Spaceflight and simulated microgravity cause a significant reduction of key gene expression in early T-cell activation." Am J Physiol Regul Integr Comp Physiol 308 6: R480-8
Healthy immune function depends on precise regulation of lymphocyte activation. During the National Aeronautics and Space Administration (NASA) Apollo and Shuttle eras, multiple spaceflight studies showed depressed lymphocyte activity under microgravity (mug) conditions. Scientists on the ground use two models of simulated mug (smug): 1) the rotating wall vessel (RWV) and 2) the random positioning machine (RPM), to study the effects of altered gravity on cell function before advancing research to the true mug when spaceflight opportunities become available on the International Space Station (ISS). The objective of this study is to compare the effects of true mug and smug on the expression of key early T-cell activation genes in mouse splenocytes from spaceflight and ground animals. For the first time, we compared all three conditions of microgravity spaceflight, RPM, and RWV during immune gene activation of Il2, Il2ralpha, Ifngamma, and Tagap; moreover, we confirm two new early T-cell activation genes, Iigp1 and Slamf1. Gene expression for all samples was analyzed using quantitative real-time PCR (qRT-PCR). Our results demonstrate significantly increased gene expression in activated ground samples with suppression of mouse immune function in spaceflight, RPM, and RWV samples. These findings indicate that smug models provide an excellent test bed for scientists to develop baseline studies and augment true mug in spaceflight experiments. Ultimately, smug and spaceflight studies in lymphocytes may provide insight into novel regulatory pathways, benefiting both future astronauts and those here on earth suffering from immune disorders.
ISSN: 1522-1490 (Electronic) 0363-6119 (Linking)
Accession Number: 25568077