Stem and precursor cell play an important role in development and regeneration. The state of these cells is regulated by biochemical substances, mechanical stimuli and cellular interactions. To estimate gravity effects We Used two types of cultured stem Cells: human mesenchymal stromal cells (hMSCS) from gone marrow and mice embryonic stem (mESC) line R1. Gravity changes were stimulated by long-term (4-7 days) slow clinorotation and leaded to decreased hMSC proliferation. changes of cell morphology and modified F-actin cytoskeleton, We did not find the shifts ill cell phenotype except for decreased expression of HLA 1 and CD105 Nit excretion of IL-6 into medium increased significantly Remodeling, of cytoskeleton started first 4h and was similar to prepoptotic changes. This data suggested the modification ill cell adhesion and possible commitment of hMSC. It was observed that expression of alkaline phosphatase by MSC ill osteogenic medium was more intensive in control. On the contrary, clinorotation did not change formation of mESC colonies and increased proliferation activity in LIF+ -medium. However the number of embryonic bodies after clinorotation was less than in static control. It is suggested that ESCs kept the viability and proliferative potential but decreased the differentiation ability after changes in gravity stimulation. (c) 2008 Published by Elsevier Ltd.
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