BACKGROUND: Leukemia inhibitory factor (LIF) is an indispensable factor for maintaining mouse embryonic stem (ES) cell pluripotency. A feeder layer and serum are also needed to maintain an undifferentiated state, however, such animal derived materials need to be eliminated for clinical applications. Therefore, a more reliable ES cell culture technique is required. METHODOLOGY/PRINCIPAL FINDINGS: We cultured mouse ES cells in simulated microgravity using a 3D-clinostat. We used feeder-free and serum-free media without LIF. CONCLUSIONS/SIGNIFICANCE: Here we show that simulated microgravity allows novel LIF-free and animal derived material-free culture methods for mouse ES cells.
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http://sfxhosted.exlibrisgroup.com/mayo?sid=OVID:embase&id=pmid:&id=doi:10.1371%2Fjournal.pone.0006343&issn=1932-6203&isbn=&volume=4&issue=7&spage=e6343&pages=&date=2009&title=PLoS+ONE&atitle=LIF-free+embryonic+stem+cell+culture+in+simulated+microgravity&aulast=Kawahara&pid=%3Cauthor%3EKawahara+Y.%3C%2Fauthor%3E&%3CAN%3E2009387611%3C%2FAN%3E