Spaceflight is known to induce vestibular and cardiovascular deconditioning. The current ESA SPIN project conducts research on vestibular and cardiovascular deconditioning after long duration spaceflight. Hereto, vestibular function and cardiovascular parameters are evaluated during centrifugation and during a tilt test in astronauts prior to and after spaceflight. The experiments are conducted using the ‘Visual and Vestibular Investigation System’. During rotation, cardiovascular and breathing parameters are recorded by means of the ‘Lifeshirt® system’ (Vivonoetics). The current analysis focuses on the cardio-respiratory response during 2 consecutive centrifugation runs, a counter clockwise (CCW) and a clockwise (CW). The RR-interval recorded postflight during the second CW rotation decreased significantly compared to the preflight data. No significant effects were observed on the parameters (amplitude, marker of vagal activity, and phase) of the respiratory sinus arrhythmia (RSA). However, the time of respiration and the amplitude of the RSA were correlated. Our preliminary results suggest a postflight recovery problem of the sympathetic nervous system after activation and show that the respiration has a large influence on the RSA amplitude.