Menu 
  • Home
  • Research on Station
        • Benefits of Research on the ISS
        • Industrial R&D
        • Current Project Pipeline
        • Researcher Interviews
      • Current RFI

        hardware

        RFI-Organs-On-Chips Research

      • Researcher Interviews

        No items found
  • Getting to Space
        • Getting to Space
        • Implementation Partners
        • ISS Hardware
        • Proposal Submission Process
        • Launch Vehicles
        • Support Services
      • Recent Posts

        No items found
      • Projects in Flight

        • Honeywell/Morehead-DM Payload Processor
        • Growth Rate Dispersion as a Predictive Indicator for Biological Crystal Samples
        • ARISS (Amateur Radio from ISS)
        • Project Meteor
        • Development and Deployment of Charge Injection Device Imagers
        View Current ISS Project Pipeline »
  • Research Library
        • ISS National Lab Research Database
        • ISS National Lab Reports
        • Web Resources
        • Research Apps
      • Recently Added Research

        • Genotype, B-vitamin status, and androgens affect spaceflight-induced ophthalmic changes
        • SUBSONIC MOTION OF A PROJECTILE IN A FLUID COMPLEX PLASMA UNDER MICROGRAVITY CONDITIONS
        • Coactivator-Dependent Oscillation of Chromatin Accessibility Dictates Circadian Gene Amplitude via REV-ERB Loading
      • Popular Tags

        • Cell Differentiation
        • Earth Observation
        • Fluid physics
        • Gene Expression
        • Human Research
        • Material science
        • Mice
        • Microbiology
        • Simulated microgravity
        • Technology demonstration
  • Make Contact
  • Home
  • Research on Station
    • Benefits of Microgravity
    • Industrial R&D
    • Current Project Pipeline
    • Research Opportunities
    • Researcher Interviews
  • Facilities & Hardware
    • ISS Hardware
    • Implementation Partners
  • Getting to Space
    • Getting to Space
    • Proposal Submission Process
    • Launch Vehicles
  • Research Library
    • Research Apps
    • Researcher Guides
    • Resources
    • Publication Database

« Go Back

Research Containing: Vcam-1

The Importance of Caveolin-1 as Key-Regulator of Three-Dimensional Growth in Thyroid Cancer Cells Cultured under Real and Simulated Microgravity Conditions

by cfynanon 22 August 2016in Biology & Biotechnology No comment

We recently demonstrated that the CAV1 gene was down-regulated, when poorly differentiated thyroid FTC-133 cancer cells formed spheroids under simulated microgravity conditions. Here, we present evidence that the caveolin-1 protein is involved in the inhibition of spheroid formation, when confluent monolayers are exposed to microgravity. The evidence is based on proteins detected in cells and their supernatants of the recent spaceflight experiment: "NanoRacks-CellBox-Thyroid Cancer". The culture supernatant had been collected in a special container adjacent to the flight hardware incubation chamber and stored at low temperature until it was analyzed by Multi-Analyte Profiling (MAP) technology, while the cells remaining in the incubation chamber were fixed by RNAlater and examined by mass spectrometry. The soluble proteins identified by MAP were investigated in regard to their mutual interactions and their influence on proteins, which were associated with the cells secreting the soluble proteins and had been identified in a preceding study. A Pathway Studio v.11 analysis of the soluble and cell-associated proteins together with protein kinase C alpha (PRKCA) suggests that caveolin-1 is involved, when plasminogen enriched in the extracellular space is not activated and the vascular cellular adhesion molecule (VCAM-1) mediated cell-cell adhesion is simultaneously strengthened and activated PRKCA is recruited in caveolae, while the thyroid cancer cells do not form spheroids.

Related URLs:
http://www.ncbi.nlm.nih.gov/pubmed/26633361

Researcher Interviews

No items found

Projects in Flight

  • Honeywell/Morehead-DM Payload Processor
  • Growth Rate Dispersion as a Predictive Indicator for Biological Crystal Samples
  • ARISS (Amateur Radio from ISS)
  • Project Meteor
  • Development and Deployment of Charge Injection Device Imagers
View Current ISS Project Pipeline »

CASIS on Twitter

Tweets by ISS_CASIS